4-Hydroxynonenal (4-HNE) ELISA Kit

Cat.#:

JL46304

Regulatory Status:

RUO

Alternative:

4-Hydroxynonenal;(2E)-4-Hydroxy-2-nonenal;2-Nonenal, 4-hydroxy-, (2E,4R); 4 HNE; 4Hydroxynonenal;4-Hydroxynonenal;4-hydroxy-trans-2-nonenal;HNE

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

0.625-40ng/mL

Sensitivity:

0.319ng/mL

Specificity:

This kit recognizes 4-HNE in samples. No significant cross-reactivity or interference between 4-HNE and analogues was observed.

Sample Type:

Serum;plasma and other biological fluids

Assay Procedure:

Research Area:

Metabolism

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| 4-HNE

4-Hydroxynonenal, or 4-hydroxy-2E-nonenal or 4-hydroxy-2-nonenal or 4-HNE or HNE, (C9H16O2), is an α,β-unsaturated hydroxyalkenal that is produced by lipid peroxidation in cells. 4-HNE is the primary α,β-unsaturated hydroxyalkenal formed in this process. It is a colorless oil. It is found throughout animal tissues, and in higher quantities during oxidative stress due to the increase in the lipid peroxidation chain reaction, due to the increase in stress events.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	40	20	10	5	2.5	1.25	0.625	0
OD	0.216	0.298	0.417	0.669	0.955	1.405	1.79	1.865

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of 4-HNE spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-101	96
Plasma(n=8)	92-105	101

| Linearity

Samples were spiked with high concentrations of 4-HNE and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	83-95	88-96
1 : 2	Average (%)	91	93
1 : 4	Range (%)	89-104	87-108
1 : 4	Average (%)	94	98